Antibodies |
ATGL Antibody |
Cell Signaling Technology |
Cat#2138; RRID:AB_2167955 |
ABHD5 monoclonal antibody (M01), clone 1F3 |
Abnova |
Cat#H00051099-M01; RRID:AB_509070 |
Anti-Perilipin-3, C terminus Antibody |
Merck KGaA |
Cat#ABS482; |
Polyclonal Rabbit anti-Mouse Immunoglobulins (HRP) |
Dako |
Cat#P0260; RRID:AB_2687969 |
Goat anti-Rabbit IgG (HRP) |
Thermo Fisher Scientific |
Cat#31460; RRID:AB_228341 LOT#QG221919 |
Goat anti-Rabbit IgG, Alexa Fluor® 594 |
Thermo Fisher Scientific |
Cat#A11037; RRID:AB_2534095 LOT#1310680 |
Critical Commercial Assays |
Triglycerides FS |
DiaSys Diagnostic Systems GmbH |
Cat#157609910023 |
Cholesterol FS |
DiaSys Diagnostic Systems GmbH |
Cat#113009910023 |
Free Cholesterol FS |
DiaSys Diagnostic Systems GmbH |
Cat#113609910930 |
Free Glycerol Reagent |
Merck KGaA |
Cat#F6428 |
NEFA-HR(2) |
Wako Chemicals GmbH |
Cat#434-91795, Cat#436-91995, Cat#270-77000 |
Bio Rad Protein Assay |
Bio-Rad Laboratories |
Cat#500-0112 |
Experimental Models: Organisms/Strains |
Mouse: WT Atglflox/flox: B6.129-Pnpla2tm1Eek
|
Erin Kershaw |
Cat#JAX: 024278; RRID: IMSR_JAX: 024278 |
Mouse: Atgl iKO Atglflox/flox/Villin-Cre(ER-T2)
|
Obrowsky et al., 2013 |
N/A |
Mouse: WT Cgi-58flox/flox: B6.129-Abhd5tm1.1Rze-flox
|
Guenter Haemmerle |
N/A |
Mouse: Cgi-58 iKO Cgi-58flox/flox/Villin-Cre(ER-T2)
|
This paper |
N/A |
Mouse: WT Atglflox/flox/Cgi-58flox/flox
|
This paper |
N/A |
Mouse: Atgl/Cgi-58 iDKO Atglflox/flox/Cgi-58flox/flox/Villin-Cre(ER-T2)
|
This paper |
N/A |
Oligonucleotides |
See Table S1 for list of all primer sequences used for qRT-PCR |
N/A |
Software and Algorithms |
Graph Pad Prism 5 |
GraphPad Software |
https://www.graphpad.com |
ImageJ |
ImageJ |
https://imagej.net |
VisiView acquisition software |
Universal Imaging, Visitron Systems |
N/A |