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A comparison of current high throughput droplet based single cell RNA-seq methods
| DropSeq | InDrops | 10X Chromium | |
|---|---|---|---|
| Year developed | 2015 | 2015 | 2016 |
| Commercial platform | No | 1CellBio | 10X Genomics |
| Full length sequence | No | No | No |
| 3’ or 5’ sequence | 3’ | 3’ | both |
| Capture efficiency | 12.8% | 90% | 65% |
|
Sensitivity (molecule detection limit) |
1e+1 | 1e+0.5 | 1e+2.5 |
| Accuracy (Pearson R) | >0.9 | >0.9 | >0.9 |
| Cell capacity per run | 10,000 | 10,000 | 80,000 |
