Table 2:
Selected candidate targets for miR-21, miR-23b and miR-664-1* that may regulate BMP-7 induced dendritic growth in sympathetic neurons
| Rno-miR-21-5p validated targets | ||
|---|---|---|
| Target mRNA | Accession ID | Go annotation |
| Programmed Cell Death 4 (Pdc4) | NM_022265 | Apoptotic signaling, BMP signaling |
| Tropomyosin 1 (Tpm1) | NM_001034069 | Actin binding, cytoskeletal protein binding |
| Potassium voltage-gated channel subfamily J member 16 (Kcnj16) | NM_053314 | regulation of transmembrane ion transport |
| Phosphatase and tensin homolog (Pten) | NM_031606 | brain morphogenesis, dendritic spine morphogenesis, neuronal differentiation |
| Pellino E3 ubiquitin protein ligase 1 (Peli1) | NM_001100565 | Protein ubiquitination, Toll signaling |
| Integrin subunit beta 1 (ITGB1) | NM_001039254 | axon extension, dendrite morphogenesis, integrin binding, laminin binding |
| ELAV like RNA binding protein 4 (Elavl4) | NM_001077651 | dendrite morphogenesis, learning |
| SKI proto-oncogene (Ski) | XM_017603153 | SMAD binding, negative regulator of BMP signaling, myelination in peripheral nervous system |
| TIMP metallopeptidase inhibitor 3 (Timp3) | NM_012886 | Negative regulation of ERK signaling, positive regulation of apoptotic signaling pathway |
| SMAD family member 7 (Smad7) | NM_030858 | negative regulator of BMP signaling |
| T-cell lymphoma invasion and metastasis 1 (Tiam1) | NM_001100558.1 | BDNF signaling pathway, dendritic spine morphogenesis, axogenesis |
| Rno-miR-21-5p predicted targets | ||
| Transforming growth factor, beta induced (Tgfbi) | NM_053802 | chondrocyte differentiation, cell adhesion, angiogenesis |
| Cytoplasmic polyadenylation element binding protein 3 (Cpeb3) | XM_017590345.1. | regulation of dendritic spine development, synaptic plasticity |
| Kruppel-like factor 3 (basic) (Klf3) | NM_001105742 | negative transcriptional regulator |
| Neurotrophin 3 (Ntf3) | NM_001270869 | axon guidance, nervous system development |
| G protein-coupled receptor 64 (Gpr64) | NM_001270872 | orphan receptor, cell signaling |
| Rho GTPase activating protein 24 (Arhgap24) | NM_001012032 | cell differentiation, GTPase activity |
| Eph receptor A4 (Epha4) | NM_001162411 | axon guidance, dendritic spine morphogenesis, neuron projection fasciculation |
| A kinase (PRKA) anchor protein 6 (Akap6) | NM_022618 | cAMP dependent PKA activity, regulation of epinephrine response |
| Jagged 1 (Jag1) | NM_019147 | Notch signaling, negative regulator of neuronal differentiation |
| Rno-miR-23b-3p predicted targets | ||
| Target mRNA | Accession ID | GO annotation |
| G protein-coupled receptor 64 (Gpr64) | NM_001270872 | G-protein receptor signaling |
| FERM domain containing 5 (Frmd5) | XM_230513 | Cytoskeletal structure, protein kinase binding, integrin binding, regulation of cellular migration, cell adhesion, cell motility |
| Protein kinase (cAMP-dependent, catalytic) inhibitor alpha (Pkia) | NM_053772 | negative regulator of cAMP-dependent protein kinase activity, negative regulator of protein import into the nucleus |
| inturned planar cell polarity protein (Intu) | XM_006232293 | smoothened signaling, cilium assembly, limb development, keratinocyte development |
| Casein kinase 1, gamma 3 (Csnk1g3) | NM_022855 | Wnt signaling, cell shape, endocytosis, protein phosphorylation |
| Latrophilin 2 (Lphn2) | NM_001190475 | synapse assembly, PDZ domain binding |
| Mastermind like 2 (Maml2) | XM_001072241 | Notch signaling pathway |
| Mediator complex subunit 12 (Med12) | NM_001193292 | Wnt signaling, protein ubiquitination, spinal cord development |
| Transforming growth factor, beta receptor III (Tgfbr3) | NM_017256 | TGF-beta signaling, BMP signaling, response to hypoxia, |
| E74-like factor 2 (Elf2) | NM_001033909 | transcriptional regulation |
| Ubiquitin-like modifier activating enzyme 6 (Uba6) | NM_001107213 | protein ubiquitination, hippocampal development, dendritic spine morphogenesis |
| Lysophosphatidic acid receptor 1 (Lpar1) | NM_053936 | inhibiting G-protein coupled signaling pathway, positive regulator of dendritic spine development, apoptosis, NF-kappaB pathway |
| SIX homeobox 4 (Six4) | NM_001106739 | negative regulator of neuronal apoptosis, regulator of synaptic growth |
| Zinc finger protein 423 (Zfp423) | NM_053583 | BMP signaling, olfactory neuron development |
| Rho-associated coiled-coil containing protein kinase 2 (Rock2) | NM_013022 | Actin cytoskeleton reorganization, dendrite morphogenesis, Rho signal transduction |
| Cysteine-rich PDZ-binding protein (Cript) | NM_019907 | Microtubule organization, regulation of post synaptic density protein 95 clustering |
| SMAD specific E3 ubiquitin protein ligase 2 (Smurf2) | NM_001107061 | SMAD binding, negative regulator of TGF-beta signaling pathway |
| Nedd4 binding protein 1 (N4bp1) | XM_001068071 | negative regulation of protein ubiquitination |
| BMP-2 inducible kinase (Bmp2k) | XM_006250707 | regulation of bone mineralization, positive regulation of Notch signaling pathway, regulation of clathrin-dependent endocytosis |
| Nedd4 family interacting protein 2 (Ndfip2) | NM_001108390 | positive regulation of protein ubiquitination, positive regulation of I-kappaB kinase/NF-kappaB signaling |
| Rno-miR-664-1* (rno-miR-664-1-5p) predicted targets | ||
| Target mRNA | Accession ID | GO annotation |
| Rho guanine nucleotide exchange factor (GEF) 15 (Arhgef15) | NM_001105789 | negative regulation of synapse maturation |
| Protein tyrosine kinase 2 beta (Ptk2b) | NM_017318 | negative regulator of apoptosis, regulator of neuronal projection, positive regulator of ROS |
| Synaptotagmin I (Syt1) | NM_001033680 | positive regulation synaptic transmission, dendrite regulation |
| Reticulon 4 receptor-like 1 (Rtn4rl1) | NM_181377 | cytokine signaling pathway, negative regulation of axon regulation |
| Serum response factor (Srf) | NM_001109302 | cell-cell adhesion, neuron migration, hippocampus development |
| Arginine-glutamic acid dipeptide (RE) repeats (Rere) | NM_053885 | dendrite morphogenesis, nerve branching morphogenesis |
| Protein tyrosine phosphatase, receptor type F (Ptprf) | NM_019249 | neurite outgrowth, axon regeneration, dendrite morphogenesis |
| sterile alpha and TIR motif containing 1 (Sarm1) | NM_001105817 | dendrite morphogenesis, neuron death |
| GLI family zinc finger 2 (Gli2) | NM_001107169 | anterior-posterior patterning, axon guidance, neuronal development, hindbrain development |
| Calcium/calmodulin-dependent protein kinase ID (Camk1d) | NM_001107365 | dendritic development, neuronal differentiation |
| SIX homeobox 3 (Six3) | NM_023990 | brain development, negative regulation of neuronal differentiation |
| 3-phosphoinositide dependent protein kinase-1 (Pdpk1) | NM_031081 | negative regulator of TGF-beta signaling, neuron apoptosis, focal adhesion assembly |
| Josephin domain containing 1 (Josd1) | NM_001025009 | protein ubiquitination (deubiquitination) |
| Ankyrin repeat domain 1 (Ankrd1) | NM_013220 | TGF-beta signaling pathway, cellular response to hypoxia, cardiac tissue morphogenesis |
| Ubiquitin specific peptidase 49 (Usp49) | NM_001136470 | protein deubiquitination |
| Glutathione peroxidase 7 (Gpx7) | NM_001106673 | response to oxidative stress, peroxidase activity |
| Nedd4 binding protein 1 (N4bp1) | XM_001068071 | negative regulation of protein ubiquitination |