Table 3:
Gene ontology analysis of genes with significantly altered expression in brain death kidneys compared to sham
| Term | # Genes Represented | Fold Enrichment | FDR |
|---|---|---|---|
| Biological Processes up-regulated in BD vs Sham: 56 known genes | |||
| negative regulation of non-canonical Wnt signaling pathway (GO:2000051) | 6 | 3 | 0.01 |
| Biological Processes down-regulated in BD vs Sham: 130 known genes | |||
| small molecule metabolic process (GO:0044281) | 32 | 4.96 | 1.94E-10 |
| organic acid metabolic process (GO:0006082) | 21 | 6.33 | 1.41E-07 |
| carboxylic acid metabolic process (GO:0019752) | 20 | 6.47 | 3.04E-07 |
| oxoacid metabolic process (GO:0043436) | 20 | 6.17 | 6.82E-07 |
| oxidation-reduction process (GO:0055114) | 21 | 4.7 | 2.62E-05 |
| organic acid catabolic process (GO:0016054) | 8 | 11.73 | 4.03E-03 |
| carboxylic acid catabolic process (GO:0046395) | 8 | 11.73 | 4.03E-03 |
| cofactor metabolic process (GO:0051186) | 11 | 6.79 | 5.82E-03 |
| alpha-amino acid metabolic process (GO:1901605) | 8 | 9.94 | 1.31E-02 |
| metabolic process (GO:0008152) | 63 | 1.65 | 1.31E-02 |
| monocarboxylic acid metabolic process (GO:0032787) | 10 | 6.93 | 1.57E-02 |
| cellular lipid metabolic process (GO:0044255) | 13 | 4.68 | 3.20E-02 |
| ion transport (GO:0006811) | 18 | 3.44 | 3.20E-02 |
| transmembrane transport (GO:0055085) | 18 | 3.36 | 4.37E-02 |
| small molecule metabolic process (GO:0044281) | 32 | 4.96 | 1.94E-10 |
Gene ontology analysis (Panther over-representation, Fisher’s Exact Test with FDR correction) of kidney gene expression showed that 56 known genes showed >2-fold increase in expression in BD (n=16) vs sham (n=6), FDR<0.05: analysis demonstrated significant enrichment in only one process. 130 known genes showed >2-fold decrease in expression in BD compared to sham, FDR<0.05: analysis demonstrated significant enrichment in processes related to metabolism.