3.

Telmisartan reduces melanoma cell viability. Percent viability of melanoma cell lines after 72 h treatment with (A) telmisartan, (B) pioglitazone and (C) losartan determined by the MTT assay presented as log (inhibitor) vs. no. rmalized response and (D) best-fit IC₅₀ values for each line, n = 4. (E) Representative graphs and (F) quantification of Annexin/PI assay for apoptosis in A375 cells after 24 h and 48 h of treatment with 50 μM telmisartan or 100 μM pioglitazone, values represent mean ± SD, ** P < 0.01, Two-way ANOVA, n = 3. (G) Flow cytometric analysis of cleaved caspase 3 in A375 cells after 48 h treatment with 50 μM telmisartan. Values represent mean ± SD, ***P < 0.001, unpaired t-test, n = 3.