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. 2019 May;16(2):234–246. doi: 10.20892/j.issn.2095-3941.2018.0284

1.

(A, B) CRC cells isogenic for <italic>BRAF V600E</italic> and with defined <italic>KRAS, PIK3CA and p53</italic> status were screened with the DTP approved oncology drug library +/- irradiation and allowed to grow for five doubling times. Cell viability was compared between irradiated and non-irradiated plates. (C) Raw data were normalized by rescaling both to the plate mean and negative controls, and quality plots contrasted. (D) Heatmaps were generated for each individual plate. ΔZ scores were calculated between irradiated and non-irradiated plates. Selection of candidate hits was based on a rank product method (see methods). Probability of false discovery was computed by permutation, with 100 permutations. (E) Example heatmap generated for one of the HTS plates. Hits were identified as drugs with a ΔZ score significantly higher than expected by chance when irradiated and non-irradiated samples were compared.

High-throughput screening of FDA approved cancer drugs to identify which drugs should be used for radiosensitisation in the context of single gene mutations in colorectal cancer.