S3.
IC50 (μM) for each drug at 0 and 4 Gy in a panel of colorectal cancer cell lines
| Cell line | 5-FU IC50 | Vorinostat IC50 | PI-103 IC50 | Olaparib IC50 | Rucaparib IC50 | Mitoxantrone IC50 |
| IC50 was calculated using Graphpad Prism following normalisation for radiation effect, and is shown in μM, with 95% confidence limits in parenthesis.
* Where the curve shape did not allow calculation of IC50 in Graphpad, IC50 was calculated manually by interpolation. * > indicates the highest concentration tested in cell lines where the IC 50 was not reached. | ||||||
| LS411 0 Gy | 24.67
(17.58 to 35.46) |
6.79
(3.99 to 11.91) |
5.23
(3.47 to 8.12) |
24.46
(15.71 to 38.89) |
*62.18 | 16.75
(12.32 to 22.98) |
| LS411 4 Gy | 21.95
(12.37 to 40.8) |
14.55
(9.69 to 22.27) |
2.76
(1.57 to 4.96) |
2.11
(1.09 to 4.18) |
1.72
(0.49 to 8.54) |
7.8
(5.63 to 10.89) |
| VACO5 0 Gy | 2.54
(1.95 to 3.35) |
3.45
(2.47 to 4.9) |
1.91
(1.05 to 3.58) |
10.5
(3.83 to 29.59) |
34.03
(21.2 to 58.03) |
3.49
(0.87 to 14.64) |
| VACO5 4 Gy | 0.99
(0.83 to 1.19) |
3.37
(2.85 to 4) |
0.48
(0.34 to 0.71) |
0.75
(0.43 to 1.3) |
3.07
(0.61 to 11.91) |
1.24
(0.56 to 2.99) |
| RKO 0 Gy | 2.51
(1.93 to 3.29) |
6.51
(3.89 to 11.26) |
1.55
(0.95 to 2.54) |
8.63
(4.32 to 17.22) |
61.23
(30.07 to 167.4) |
9.75
(6.21 to 15.44) |
| RKO 4 Gy | 1.15
(0.73 to 1.90) |
2.14
(1.2 to 4.15) |
0.33
(0.20 to 0.57) |
0.35
(0.15 to 0.78) |
0.3
(0.03 to 1.59) |
2.9
(1.59 to 5.46) |
| HT29 0 Gy | 9.12
(6.67 to 12.66) |
3.47
(2.42 to 5.03) |
* >20 | 17.93
(4.14 to 75.29) |
51.82
(33.61 to 86.34) |
6.58
(1.24 to 78.99) |
| HT29 4 Gy | 6.6
(5.24 to 8.36) |
4.18
(2.6 to 6.94) |
* 12.94 | 2.21
(1.25 to 3.55) |
5.48
(2.49 to 11.75) |
3.06
(0.88 to 12) |
| OXCO4 0 Gy | 16.71
(14.13 to 19.85) |
6.09
(3.69 to 10.41) |
2.42
(1.82 to 3.24) |
26.88
(16.82 to 43.79) |
13.11
(10.42 to 16.58) |
0.89
(0.61 to 1.33) |
| OXCO4 4 Gy | 9.45
(7.92 to 11.32) |
3.82
(2.69 to 5.49) |
1.13
(0.87 to 1.47) |
6.07
(4.73 to 7.82) |
2.5
(1.74 to 3.61) |
0.59
(0.44 to 0.78) |
| CW2 0 Gy | 20.19
(15.17 to 27.24) |
4.49
(2.9 to 7.08) |
4.97
(3.14 to 8.17) |
17.05
(6.48 to 44.79) |
36.91
(30.48 to 45.16) |
19.81
(11.2 to 35.76) |
| CW2 4 Gy | *20.1 | 5.33
(3.59 to 7.99) |
4.21
(1.49 to 14.87) |
* >20 | * >30 | 21.02
(12.56 to 186) |
| DLD1 0 Gy | 8.6
(6.77 to 10.99) |
6.26
(3.07 to 13.72) |
1.69
(0.94 to 3.08) |
* >100 | *30.41 | 4.08
(1.49 to 12.46) |
| DLD1 4 Gy | 7.78
(5.26 to 11.81) |
3.25
(2.02 to 5.42) |
0.52
(0.29 to 0.95) |
1.74
(0.89 to 3.5) |
0.44
(0.15 to 2.1) |
1.9
(1.33 to 2.72) |
| CCK81 0 Gy | 29.85
(23.48 to 38) |
10.4
(5.37 to 21.34) |
1.27
(0.92 to 1.76) |
>100 | *48.51 | *16.51 |
| CCK81 4Gy | 20.77
(16.64 to 26.01) |
7.84
(4.13 to 15.86) |
1.07
(0.81 to 1.44) |
13.05
(7.62 to 22.53) |
45.05
(11.83 to 105.1) |
22.6
(11.62 to 60.7) |
| C10 0 Gy | 43.38
(31.27 to 60.93) |
2.05
(0.82 to 6.16) |
0.98
(0.45 to 2.19) |
* >100 | 23.7
(20.64 to 222.2) |
3.92
(1.84 to 8.96) |
| C10 4 Gy | 39.86
(17.15 to 101.3) |
10.21
(1.3 to 74.5) |
0.53
(0.27 to 1.17) |
* >100 | 22.4
(6.87 to 130) |
2.18
(1.14 to 4.29) |
| SW403 0 Gy | 1.31
(0.86 to 2.02) |
17.71
(7.57 to 49.28) |
* >20 | 6.18
(1.46 to 26.89) |
40.51
(27.91 to 61.56) |
2.17
(0.94 to 5.27) |
| SW403 4 Gy | 0.73
(0.49 to 1.09) |
7.28
(4.53 to 12.05) |
10.81
(5.35 to 27.22) |
0.85
(0.28 to 2.46) |
12.39
(5.71 to 26.75) |
1.46
(0.66 to 3.41) |
| COLO678 0 Gy | 85
(37.7 to 197.4) |
8.68
(1.84 to 64.49) |
2.3
(1.08 to 5.15) |
* >200 | * 48.67 | 5.96
(2.98 to 12.18) |
| COLO678 4 Gy | 81.5
(70.58 to 129.3) |
*5.5 | 2.24
(1.09 to 4.85) |
* >200 | * 45.79 | 20.31
(12.29 to 34.44) |
| SW1222 0 Gy | 10.58
(5.80 to 20.42) |
41.93
(16.03 to 134) |
* >20 | 16.72
(9.97 to 28.91) |
9.78
(4.56 to 22.31) |
2.76
(1.63 to 4.8) |
| SW1222 4 Gy | 3.23
(2.19 to 4.75) |
4.07
(2.98 to 5.63) |
0.75
(0.63 to 0.90) |
0.42
(0.32 to 0.56) |
* 0.32 | 0.73
(0.32 to 1.83) |
| HCA7 0 Gy | 27.64
(22.63 to 33.87) |
1.29
(0.93 to 1.80) |
2.95
(1.71 to 5.26) |
3.99
(3.16 to 5.05) |
48.51
(35.36 to 68.98) |
1.93
(0.57 to 6.89) |
| HCA7 4 Gy | 19.82
(16.49 to 23.89) |
0.82
(0.70 to 0.97) |
1.14
(0.79 to 1.66) |
0.24
(0.18 to 0.32) |
0.36
(0.19 to 0.66) |
0.75
(0.24 to 2.53) |
| HT55 0 Gy | 10.53
(7.91 to 14.17) |
2.11
(1.51 to 3) |
2.87
(0.95 to 9.99) |
41.07(4.96 to 28.01) | 12.2
(8.31 to 18.19) |
1.26
(0.86 to 1.85) |
| HT55 4 Gy | 12.03
(8.91 to 16.48) |
3.14(1.99 to 5.10) | 2.58
(1.85 to 3.63) |
7.88
(0.88 to 3.66) |
1.47
(0.4 to 5.18) |
1.27
(0.79 to 2.08) |
| C99 0 Gy | 3.34
(2.11 to 5.72) |
3.53
(1.8 to 7.46) |
23.77
(8.94 to 31.98) |
14.01
(4.11 to 53.54) |
39.28
(21.29 to 81.87) |
1.15
(0.58 to 2.31) |
| C99 4 Gy | 4.44
(2.37 to 8.86) |
3
(1.66 to 5.92) |
0.97
(0.30 to 3.48) |
0.44
(0.22 to 0.87) |
14.2
(0.16 to 18) |
0.49
(0.27 to 0.92) |
| Cell line | AZD-7762 IC50 | PF4777 IC50 | AZD-6244 IC50 | Trametinib IC50 | Vemurafenib IC50 | |
| LS411 0 Gy | 2.69
(1.5 to 6.50) |
3.84
(2.91 to 5.08) |
11.92
(5.13 to 39.4) |
2.03
(0.08 to 25.26) |
58.81
(30.19 to 144.8) |
|
| LS411 4 Gy | 0.41
(0.25 to 0.69) |
1.49
(1.06 to 1.83) |
6.94
(2.62 to 24.83) |
1.81
(0.006 to 48.6) |
20.39
(4.63 to 210.7) |
|
| RKO 0 Gy | 0.02
(0.015 to 0.03) |
0.47
(0.31 to 0.71) |
*148.75 | 0.09
(0.03 to 0.3) |
15.14
(4.37 to 57.2) |
|
| RKO 4 Gy | 0.005
(0.004 to 0.008) |
0.19
(0.15 to 0.25) |
4.62
(0.74 to 46.47) |
0.03
(0.01 to 0.07) |
4.57
(0.99 to 29.93) |
|
| VACO5 0 Gy | 0.05
(0.03 to 0.11) |
1.5
(1.06 to 2.16) |
14.81
(7.95 to 29.18) |
0.01
(0.007 to 0.017) |
9.37
(6.57 to 13.45) |
|
| VACO5 4 Gy | 0.01
(0.004 to 0.02) |
0.28
(0.23 to 0.34) |
7.08
(4.28 to 11.86) |
0.003
(0.003 to 0.004) |
3.86
(2.6 to 5.84) |
|
| HT29 0 Gy | 0.03
(0.02 to 0.06) |
4.08
(2.58 to 6.89) |
2.34(1.02 to 5.62) | 0.02
(0.01 to 0.04) |
13.1
(5.49 to 32.24) |
|
| HT29 4 Gy | 0.01
(0.003 to 0.03) |
1.57
(1.03 to 2.45) |
1.87(0.62 to 6.39) | 0.01
(0.007 to 0.02) |
11.76
(6.68 to 20.96) |
|
| OXCO4 0 Gy | 2.14
(1.39 to 3.73) |
1.54
(1.08 to 2.22) |
3.04
(1.76 to 5.35) |
*0.15 | 14.57
(10.14 to 21.19) |
|
| OXCO4 4 Gy | 0.17
(0.13 to 0.22) |
0.76
(0.54 to 1.09) |
0.82
(0.33 to 2.4) |
*0.06 | 10.6
(4.09 to 28.44) |
|
| CW2 0 Gy | 2.16
(1.17 to 5.32) |
26.75
(21.9 to 32.78) |
1.72
(0.47 to 9.39) |
0.46
(0.2 to 1.24) |
*53.05 | |
| CW2 4 Gy | * >2 | 20.75
(6.06 to 71.96) |
* >10 | 0.18
(0.076 to 0.44) |
*49.07 | |
| DLD1 0 Gy | 0.14
(0.1 to 0.21) |
* 15.02 | * >20 | * >1 | *66.41 | |
| DLD1 4 Gy | 0.02
(0.01 to 0.05) |
5.46
(2.36 to 12.91) |
* >20 | 0.08
(0.02 to 0.59) |
33.24
(14.01 to 84.84) |
|
| CCK81 0 Gy | 0.75
(0.43 to 1.42) |
* >10 | * >20 | *>1 | * >160 | |
| CCK81 4Gy | 0.11
(0.08 to 0.17) |
* >10 | * >20 | * >1 | * >160 | |
| C10 0 Gy | 0.12
(0.1 to 0.15) |
*10.08 | *25.69 | 0.68
(0.26 to 3.31) |
53.16
(30.73 to 99.45) |
|
| C10 4 Gy | 0.12
(0.08 to 0.2) |
* >10 | * >20 | 0.29
(0.13 to 0.74) |
48.79
(23.05 to 119.8) |
|
| SW403 0 Gy | 0.26
(0.18 to 0.37) |
0.79
(0.44 to 1.41) |
6.45
(3.02 to 15.7) |
*2.03 | * >80 | |
| SW403 4 Gy | 0.13
(0.1 to 0.16) |
0.37
(0.22 to 0.62) |
1.71
(1.08 to 2.76) |
* 1.68 | * >80 | |
| COLO678 0 Gy | * >2 | *25.14 | 1.12
(0.72 to 1.78) |
0.006
(0.005 to 0.008) |
* >80 | |
| COLO678 4 Gy | * >2 | *25.39 | 1.47
(1.07 to 2.04) |
0.005
(0.002 to 0.01) |
* >80 | |
| SW1222 0 Gy | 0.07
(0.05 to 0.1) |
6.26
(4.02 to 10.64) |
3.75
(0.64 to 45.5) |
0.23
(0.09 to 0.69) |
* >160 | |
| SW1222 4 Gy | 0.02
(0.02 to 0.02) |
1.19
(0.76 to 1.91) |
0.61
(0.43 to 0.87) |
0.04
(0.02 to 0.07) |
39.5
(27.01-57.36) |
|
| HCA7 0 Gy | 0.06
(0.01 to 0.47) |
2.37
(1.76 to 3.26) |
* >20 | 0.41
(0.21 to 0.89) |
196.8
(178.93 to 231) |
|
| HCA7 4 Gy | 0.01
(0.00 to 0.14) |
0.42
(0.36 to 0.49) |
*18.77 | 0.15
(0.09 to 0.24) |
116.7
(64.29 to 256.3) |
|
| HT55 0 Gy | 0.06
(0.05 to 0.09) |
0.97
(0.73 to 1.28) |
1.55
(0.28 to 5.02) |
0.08
(0.03 to 0.29) |
49.39
(15.7 to 169.4) |
|
| HT55 4 Gy | 0.02
(0.01 to 0.02) |
0.37
(0.3 to 0.47) |
1.55
(0.41 to 3.33) |
0.05
(0.03 to 0.09) |
50.77
(22.77 to 132) |
|
| C99 0 Gy | 0.12
(0.07 to 0.22) |
3.75
(1.92 to 8.38) |
1.27
(0.32 to 5.5) |
0.01
(0.007 to 0.023) |
* >160 | |
| C99 4 Gy | 0.34
(0.07 to 2.32) |
1.43
(0.32 to 7.72) |
0.38
(0.16 to 1.08) |
0.004
(0.002 to 0.007) |
* >160 | |