Table 1. Comparison of assay characteristics.
| Characteristics | CLIA-IGRA | QFT-GIT assay |
|---|---|---|
| Peptide used | ESAT-6, CFP-10, TB7.7 | ESAT-6, CFP-10, TB7.7 |
| Incubation temperature and time | 37℃, 16–24 hours | 37℃, 16–24 hours |
| Minimum sample volume required | 50 µL | 50 µL |
| Centrifugation conditions for plasma harvesting | 2,000 × g, 5 minutes | 2,000–3,000 × g, 15 minutes |
| Time to obtain results* | 15 minutes | 2 hours 30 minutes |
| Analytical measurement range | 0–100 IU/mL | 0–10 IU/mL |
| Number of samples per run | 2 samples/machine (8 wells) | 29 samples/ microplate (96 wells) |
| Assay type | CLIA | ELISA |
| Assay platform | Automated | Automated/Manual |
*Time after incubation.
Abbreviations: TB, tuberculosis; ESAT-6, early secreted antigenic Target 6; CFP-10, culture filtrate protein 10; CLIA, chemiluminescence immunoassay; CLIA-IGRA, chemiluminescent immunoanalyzer-based IGRA; QFT-GIT, QuantiFERON-TB Gold In-Tube.