Fig. 2.

VEGFA protein levels and effects of VEGFA administration on hepatic damage and function, liver regeneration 24 h after surgery, and survival rate in Ob Zucker rats. A Protein levels of VEGFA in liver. Representative Western blots at the top and densitometric analysis at the bottom. B Representative photomicrographs of VEGFA immunohistochemical positivity in liver (× 20). C Hepatic injury (plasma AST, ALT, and GLDH levels; damage score and representative photomicrographs of necrosis (× 10). D Hepatic function (ALP and bilirubin levels). E Endothelial cell damage (vWF and HA levels). F Lipid peroxidation (MDA levels) and neutrophil accumulation (MPO levels). G Hepatic regeneration (percentage of Ki67-positive-hepatocytes and representative photomicrographs of Ki67 immunohistochemical positivity (× 10); cyclin D1, E, and A levels). Survival of Ob Zucker animals was monitored (postoperative day 14) (G). *P < 0.05 versus sham; +P < 0.05 versus PH+I/R