Fig. 6.

Underlying action mechanisms of VEGFA in non-steatotic livers of Ln Zucker rats 24 h after surgery. A Protein levels of VEGFR2, Id1, and Wnt2. Representative Western blots at the top and densitometric analysis at the bottom. B Hepatic injury (plasma AST, ALT, and GLDH levels; damage score and representative photomicrographs of necrosis (× 10)). C Hepatic function (ALP and bilirubin levels). D Endothelial cell damage (vWF and HA levels). E Lipid peroxidation (MDA levels) and neutrophil accumulation (MPO levels). F Hepatic regeneration (percentage of Ki67 positive-hepatocytes and representative photomicrographs of Ki67 immunohistochemical positivity (× 10); cyclin D1, E, and A levels). G Protein levels of PI3K and p-AKT. Representative Western blots at the top and densitometric analysis at the bottom. Survival of Ln Zucker animals, per group was monitored (postoperative day 14) (Fig. 5F). *P < 0.05 versus sham; +P < 0.05 versus PH+I/R; #P < 0.05 versus PH+I/R+VEGFA