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. 2019 Aug 28;9:12465. doi: 10.1038/s41598-019-48875-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Exogenous expression of Golgin45.D171A mutant results in intercisternal fusion. (A) Both WT Golgin45 and Golgin45.D171A mutant significantly co-localized with endogenous Syntaxin5 in HeLa cells. GT-GFP was co-expressed as a Golgi marker. Bar = 10 μm (B) Representative EM photo showing that expression of Golgin45.D171A mutant, but not the Golgin45 WT, results in frequent intercisternal fusions at the Golgi in HeLa cells. Insets show a magnified view of the boxed area. Intercisternal fusions are marked by black arrow heads. (scale bar = 1 μm) M = mitochondria, N = nucleus, G = Golgi.