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. 2019 Aug 28;9:12465. doi: 10.1038/s41598-019-48875-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Electron tomography of the Golgi showing extensive intercisternal fusion induced by Golgin45.D171A expression. (A) Golgin45.D171A mutant fails to restore normal Golgi morphology in GRASP65/55-depleted cells, whereas expression of WT Golgin45 successfully restores normal Golgi stack morphology, as shown previously. (B) EM tomography tilt series showing extensive intercisternal fusion occurring throughout the Golgi stack in HeLa cells treated with GRASP65/55 siRNAs for 48 hours, followed by overnight expression of Golgin45.D171A mutant. Insets show the boxed areas along with 3D reconstruction of inter-cisternal fusion sites. Black arrow heads indicate intercisternal fusions.