Figure 5.
Depletion of Golgin45 leads to a significant reduction in the number of cisternae per Golgi stack (A–E) Representative EM photos showing HeLa Golgi stacks in Golgin or GRASP single knowndown cells. HeLa cells were treated with siRNAs against scrambled control (A); GM130 (B); Golgin45 (C); GRASP65 (D); GRASP55 (E) for 96 hours and processed for EM study, as described previously. (F) Bar graph summarizing the average number of cisternae per stack in single KD cells with standard deviation; (G) Table summarizing the results and the number of Golgi stacks used in the quantification for the number of cisternae per stack. (scale bar = 1 μm) M = mitochondria, N = nucleus, G = Golgi.
