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. 2019 Aug 28;9:12453. doi: 10.1038/s41598-019-47946-3

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Gross appearance, H&E staining and Alcian blue staining of serum-free medium cultured chondrocyte collagen microspheres of different time points. Collagen microspheres were cultured in serum-free medium for different period of time. H&E staining (F–H) and Alcian blue staining (I–K) of collagen microspheres cultured in serum-free medium for different period of time. The change in diameter of microsphere cultured with serum-free medium (L), from 4 independent experiments, each with diameter measurement of 30 microspheres at each time points.