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. 2019 Aug 28;9:12456. doi: 10.1038/s41598-019-48860-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Dynein is required for ER concentration around centrosomes in late interphase. (a) Representative images of control (upper panel) and Dhc64C RNAi (lower panel) spermatocytes co-expressing GFP-tubulin (green) and RFP-KDEL (red) during late interphase. (b) Representative images of control (upper panel) and Dhc64C RNAi (lower panel) spermatocytes co-expressing Ana1-tdTomato, H2A-RFP (red) and GFP-PDI (green) during late interphase. Dotted lines outline individual cells, and filled arrowheads point to visible centrosomes. Open arrowheads indicate the approximate location of centrosomes that are out of the focal plane.