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. 2019 Aug 28;9:12456. doi: 10.1038/s41598-019-48860-4

Figure 5.

Figure 5

Ncd is not required for astral MT association of the ER in M-phase. (a) Representative images of a ncd RNAi expressing spermatocyte with GFP-tubulin (green) and RFP-KDEL (red) from late interphase (−50 min) through metaphase (+20 min) of meiosis (times relative to NEB, lower left of images). Note the clear association of ER with astral MTs at metaphase (compare to Fig. 1a). (b) Representative images of a spermatocyte co-expressing ncd and Dhc64C RNAi, with GFP-tubulin (green) and RFP-KDEL (red), from late interphase (−32 min) through metaphase (+50 min) of meiosis. Note the similarities to cells with Dhc64C RNAi alone (compare to Fig. 2b). Filled arrowheads indicate visible centrosomes, and open arrowheads indicate the approximate location of centrosomes that are out of the focal plane.