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. 2019 Aug 28;9:12456. doi: 10.1038/s41598-019-48860-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Klp61F is not required for astral MT association of the ER in M-phase. (a) Representative images of a Klp61F RNAi expressing spermatocyte with GFP-tubulin (green) and RFP-KDEL (red) from late interphase (−46 min) through metaphase (+36 min) of meiosis (times relative to NEB, lower left of images). Note the collapse of the spindle with movement of the two spindle poles toward one another during metaphase (+36 min). (b) Representative images of a spermatocyte co-expressing Klp61F and Dhc64c RNAi with GFP-tubulin (green) and RFP-KDEL (red) from late interphase (−48 min) through metaphase (+98 min) of meiosis. Note the similarities to Dhc64C RNAi alone (compare to Fig. 2b). Filled arrowheads indicate visible centrosomes, and open arrowheads indicate the approximate location of centrosomes that are out of the focal plane.