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. 2019 Aug 28;9:12493. doi: 10.1038/s41598-019-48886-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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PDE8A expression in ovarian follicular cells. (A) PDE8A transcript detection by RT-PCR in ovarian follicular cells using PDE8A-F and PDE8A-R primers. Lane 1: PCR conducted without template; Lane 2: granulosa cells; Lane 3: COC; Lane 4: cumulus cells; Lane 5: oocytes. Amplifications were conducted on three biological replicates (n = 3). (B) Western blot of PDE8A in ovarian follicular cells using anti-PDE8A (Proteintech). Lane 1: granulosa cells; Lane 2: COC; Lane 3: cumulus cells; Lane 4: oocytes. Protein molecular mass markers are indicated on the right (kDa) (one representative replicate of 3). Immunohistochemical localization of PDE8A in (C) early antral follicle and (D) COC (n = 3). Immunolabelling with anti-PDE8A (Proteintech) was observed with a green signal. The DNA signal was obtained with DAPI in blue. PDE8A signal was calibrated with the non-specific IgG signal. Oocyte diameter is 100 µm.