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. 2019 Aug 28;9:12493. doi: 10.1038/s41598-019-48886-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Western blot of granulosa cell protein extracts (lane 1), mitochondrial fractions from granulosa cells (lane 2), or COCs (lane 3), showing the relative abundance of PDE8A (Proteintech), voltage-dependent anion channel (VDAC, Cell signaling technology Inc.) and cytochrome c oxidase subunit IV (COXIV, Cell signaling technology Inc.). Protein molecular mass markers are indicated on the right (kDa).