Figure 3.

Modularity of the Dynamic Stimulation System

(A) A variant device with four inlets to the mixer for simultaneous control of multiple distinct stimuli. Each inlet is connected to reservoirs containing growth medium or different stimuli.

(B) Example experiment using reservoirs with Alexa 594- and Alexa 647-conjugated BSA (A594 or A647, respectively, in A) connected to two of the mixer inlets. The other inlets are connected to reservoirs with Medium only (M). Resulting fluorescence measured at the same point in the “E” band of the cell culture device shows that out-of-phase oscillations can be achieved.