Figure 2. Surgical procedure. (A) Bone defects were made in the rabbit calvarium. A circular defect with a diameter of 6 mm was formed in the skull. (B) The groups were designated as follows; group 1: β-TCP/HA+1×105 MSCs, group 2: type I collagen membrane+1×105 MSCs, group3: β-TCP/HA+type I collagen membrane+1×105 MSCs and group 4: β-TCP/HA+type I collagen chipped membrane+1×105 MSCs. (C) A Teflon sheet was applied and titanium screws were placed before suturing. The periosteum and scalp were replaced with non-absorbable sutures. (D) Harvesting of the grafted tissue was done at 24 hours after surgery.
β-TCP/HA: β-tricalcium phosphate/hydroxyapatite, MSCs: mesenchymal stem cells.