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YAM2478 (WT) and YAM2826 (Dcr1-GFP) cells were grown to mid-log phase in CSM, at 25°C. After fixation of cells, the subcellular localization of Dcr1-GFP was performed using immunofluorescence using GFP nanobody (see the Materials and Methods section). DAPI staining was used to visualize DNA. Scale bars: 1 μm.
