Fig. 5.
(A) The semi-quantitative RT-PCR demonstrates the gene expression changes for Bax, Bcl-2, p53 and β-actin (B) Densitometry analysis of intrinsic apoptotic related gene expressions of A549 cells exposure to AgNPs for 24, 48 and 72 h. The results shown here are representative of three independent experiments. (C) Immunoblot showing the expression of apoptotic responsive proteins such as cyt c, caspases -9, caspases -3 and β-actin (D) Densitometry quantification of apoptotic protein expression. The results exhibited here are representative of three independent experiments. β-actin expression was used as a loading control.