FIGURE 6.

Frequency of interleukin (IL)-13⁺ CD4⁺ T-cells and IL-13⁺ innate lymphoid cells (ILCs) from severe therapy-resistant asthma (STRA) patient peripheral blood are significantly reduced after steroids in vitro. Frequency of IL-13 expressing a) ILC2s (CD127⁺CD45⁺Lin^neg) and b) CD4 T-cells (CD45⁺CD3⁺CD4⁺) after in vitro treatment of peripheral blood mononuclear cell cultures with house dust mite (HDM) and/or budesonide (Bude) for 72 h. c) IL-13 protein levels in culture supernatants. STRA n=9. Wilcoxon matched pairs test. *: p<0.05, **: p<0.01.