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. 2019 May 6;8(3):379–405. doi: 10.1016/j.jcmgh.2019.04.015

Figure 19.

Figure 19

xCT blockade with sulfasalazine leads to increased ROS in chief cells without inflammation. Sections from DMP-777–treated (n = 3) and DMP-777 + sulfasalazine–treated (n = 3) C57Bl/6J mice killed after 3 days of L635 treatment were incubated with ROS indicators DHE and CellROX. (A) Representative images of DHE staining with oxidized DHE (DHE-ox) (red), F-actin marker phalloidin (green), and unoxidized DHE (blue). Scale bars: 100 μm. Magnified inset of chief cell region (right). (B) Relative fluorescence intensity of nuclear DHE-ox (P = .02∗). (C) Representative images of CellROX green staining with zymogenic granule marker GIF (red), CellROX Green Reagent (green), with nuclear counterstain Hoechst (blue). Scale bars: 100 μm. Magnified inset of chief cell region (right). (D) Relative fluorescence intensity of CellROX in GIF-positive cells (P = .02∗). Statistical significance was determined by unpaired Student t test.