Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Aug 22;10:2001. doi: 10.3389/fimmu.2019.02001

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2019 Bari, Granzin, Tsang, Roy, Krueger, Orentas, Schneider, Pfeifer, Moeker, Verhoeyen, Dropulic and Leung.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

VSV-G pseudotyped LV efficiently transduces T cells but not NK cells. Magnetic separation was used for isolation of T cells (CD3⁺) and NK cells (CD3⁻/CD56⁺) from PBMC (A). Purified T and NK cells were cultivated for 2 days, then transduced with different titers of VSV-G pseudotyped LV at MOI 10 for GFP expression or left non-transduced as a control. Exemplary dot plots from 1 donor are shown for MOI 10 (B). NK and T -cells were transduced with different MOI (C). The expression of VSV-G receptor LDL-R was measure at day 0 and 2 days after activation (D). The results shown are average from at least three different donors. ***P ≤ 0.0005.