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. 2019 Aug 22;10:2001. doi: 10.3389/fimmu.2019.02001

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Copyright © 2019 Bari, Granzin, Tsang, Roy, Krueger, Orentas, Schneider, Pfeifer, Moeker, Verhoeyen, Dropulic and Leung.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Activated but not naive NK and T cells expressed the receptor for baboon envelope ASCT2. NK cells were activated by growing them in NK-MACS medium (Miltenyi Biotec GmbH) with IL-2 and IL-15 for 2 days. T cells were activated with transact beads. (A) Naive and activated NK and T cells as well as NK 92 cell line were lysed in RIPA lysis buffer and subjected to westernblot analysis using antibody against ASCT2. GAPDH was used as loading control. (B) NK92 cell line was transduced with BaEV and VSV-G pseudotyped lentiviral vector containing GFP. Percentage of transduction at MOI 10 are shown in upper part of the figure. Transduction at different MOI are shown in lower part of the figure. (C) Non-transduced and transduced activated NK and T cells were perform using BaEV-LVs containing GFP. Comparison of activated NK and T cells transduction with different MOI of BaEV-LVs are shown in lower part of the figure.