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. 2019 Aug 22;10:2001. doi: 10.3389/fimmu.2019.02001

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Copyright © 2019 Bari, Granzin, Tsang, Roy, Krueger, Orentas, Schneider, Pfeifer, Moeker, Verhoeyen, Dropulic and Leung.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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CX3CR1⁻ subset of NK cells is highly proliferative and transducible. (A) Different NK cell subsets were separated based on their CX3CR1 expression using MCAS sorting prior to transduction and culture. (B) Average of three independent separation and expression of CX3CR1 in different separated NK cell population. (C) Representative figure for proliferation of unsorted NK cells, CX3CR1⁺ subset, and CX3CR1⁻ subset of NK cells are shown. (D) Average of the independent proliferation experiments. (E) Representative transduction efficiency of unsorted NK cells, CX3CR1⁺ subset, and CX3CR1⁻ subset of NK cells. (F) Average of three independent transduction experiment are shown. Unsorted, total NK cell isolated from PBMC; Enriched, CX3CR1 positive NK cells are sorted; Depleted, CX3CR1 negative NK cell subset collected from sorting. *P ≤ 0.05, ***P ≤ 0.0005.