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. 2019 Aug 22;10:2001. doi: 10.3389/fimmu.2019.02001

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Copyright © 2019 Bari, Granzin, Tsang, Roy, Krueger, Orentas, Schneider, Pfeifer, Moeker, Verhoeyen, Dropulic and Leung.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Transduction of NK cells with BaEV pseudotyped vector allows high expression of CD19-CAR and increased CAR specific cytotoxicity. Activated NK cells were transduced with BaEV pseudotyped LV for expression of a CAR directed against CD19, or the cells were left non-transduced as a control. The aCD19-CAR expression of transduced cells (black) or non-transduced cells (gray) was measured by FACS and is shown in an exemplary histogram for 1 donor (A left) and a bar chart for mean and SD of 4 donors from different experiments (A right). Different target cells were stained for CD19 (black) or an isotype control (white) and analyzed by flow cytometry (B). Killing of three target cell lines by aCD19-CAR expressing NK cells (black) or non-transduced NK cells (gray) from four different donors were analyzed at different effector-to-target ratios in different experiments and mean and SD are displayed (C). *P ≤ 0.05, **P ≤ 0.005, ***P ≤ 0.0005.