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. 2019 Aug 22;10:1903. doi: 10.3389/fmicb.2019.01903

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Copyright © 2019 Fernández-Juárez, Bennasar-Figueras, Tovar-Sanchez and Agawin.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Structural analysis of PhoD and PhoU of Halothece sp. PCC 7418. (A) Predicted structure of PhoD of Halothece sp. PCC 7418 is represented in red and aligned with the crystal structure 2YEQ that is displayed in blue cyan. (B) Active center of PhoD of the test bacterium showing all the aminoacids involved in coordination with two Ca²⁺ and Fe³⁺ ions. (C) Cluster 1 of PhoU, trinuclear metal site with Fe, between α5 and α6. (D) Cluster 2 of PhoU, tetranuclear metal site with three Fe and one Ni, between α2 and α3. Black amino acids are from predicted PhoU and red amino acids are from 4Q25. All the structures were represented with Pymol.