Figure 2.

Viral RNA transcripts in four tumour lesions of four individual rabbits IV infected by CRPV virions through marginal ear vein injection. Total RNA isolated from each tumour and depleted of ribosomal RNA was analyzed by RNA-seq. By mapping the RNA-seq raw reads to the newly arranged linear CRPV genome starting from nt 7421 and ending at nt 7420 and by uploading these uniquely mapped viral RNA reads to the Integrative Genomics Viewer (IGV) program to visualize reads coverage profile along with the CRPV genome, three major coverage peaks were visualized in the E6, E7 and E1^E4 regions among all tumour tissues.