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. 2019 Jul 25;8(1):1108–1121. doi: 10.1080/22221751.2019.1637072

Figure 4.

Figure 4.

Representative host genes with differential expression in skin tumours induced by both routes of CRPV infections. (A) Heat map showing the expression of 9 selected host genes chosen based on their expression abundance and cellular functions. A colour scale bar represents relative gene expression level within centred rows. Unit variance scaling has been applied to rows. Both rows and columns are clustered using Euclidean distance and complete linkage. (B) Verification of differentially expressed rabbit genes in RNA-seq results by real-time RT-qPCR. Consistent with RNA-seq data, SLN, TAC1, MYH8, and PGAM2 were down-regulated in both CRPV blood infection (Animal #9, #11, and #12) and CRPV skin infection (Animal#6, #7, and #8) animals relative to those in normal control animals, whereas SDRC7, KRT16, S100A9, IL36G, and FABP9 were up-regulated in both CRPV skin infection and CRPV blood infection animals compared to normal controls. The Y-axis indicates relative gene expression levels calculated by 2-ΔΔCт and the X-axis indicate the different samples. NC, gene expression in the normal tissue, was set to 1 after normalization to GAPDH. (C) Western blot analysis of representative samples from normal skin and warts induced by cutaneous or intravenous (IV) CRPV infection for the expression of S100A9 and APOBEC2. Cellular β-tubulin served as a loading control.