Table 2 |.

Cytosolic and mitochondrial-free NADPH, NADP⁺, NADPH/NADP⁺ ratio, NADH, NAD⁺, and NADH/NAD⁺ ratio in mammalian cells.

	Cytosol	Mitochondria	reference
NADPH	~3.1 μM	~37 μM	8
NADP+	~9.3–200 nM	~185 nM	a
NADPH/NADP+ ratio	15–333	200	9,31,39
NADH	~0.12 μM	~30 μM	24
NAD+	~106 μM	~230 μM	37
NADH/NAD+ ratio	0.001–0.0167	0.1–0.25	23,57

^aCytosolic and mitochondrial-free NADP⁺ was calculated from the free NADPH level⁸ and free NADP⁺/NADPH ratio^9,31,39 in mammalian cells.