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. Author manuscript; available in PMC: 2019 Oct 1.
Published in final edited form as: Nat Protoc. 2018 Oct;13(10):2362–2386. doi: 10.1038/s41596-018-0042-5

Table 3.

Overview of plasmids used in this study.

Name Target Excitation (nm) Emission (nm) Targeting Cell type Source
pRSETB-iNap1–4 NADPH 420/500 515 Cytosol E. coli Ref.8
pcDNA3.1/Hygro(+)-cyt-iNap1 NADPH 420/500 515 Cytosol Mammalian Ref.8
pcDNA3.1/Hygro(+)-nes-iNap1 NADPH 420/500 515 Nucleus-excluded Mammalian Ref.8
pLVX-cyt-iNap1 NADPH 420/500 515 Cytosol Mammalian Ref.8
pTol2-iNap1 NADPH 420/500 515 Cytosol Zebrafish Ref.8
pcDNA3.1/Hygro(+)-mit-iNap3 NADPH 420/500 515 Mitochondria Mammalian Ref.8
pcDNA3.1/Hygro(+)-cyt-iNapc pH 420/500 515 Cytosol Mammalian Ref.8
pcDNA3.1/Hygro(+)-nuc-iNapc pH 420/500 515 Nucleus Mammalian Ref.8
pcDNA3.1/Hygro(+)-mit-iNapc pH 420/500 515 Mitochondria Mammalian Ref.8
pLVX-cyt-iNapc pH 420/500 515 Cytosol Mammalian Ref.8
pTol2-iNapc pH 420/500 515 Cytosol Zebrafish Ref.8
pcDNA3.1/Hygro(+)-cyt-SoNar NADH/NAD+ ratio 420/500 518 Cytosol Mammalian Ref.22
pcDNA3.1/Hygro(+)-nuc-SoNar NADH/NAD+ ratio 420/500 518 Nucleus Mammalian Ref.22
pLVX-cyt-SoNar NADH/NAD+ ratio 420/500 518 Cytosol Mammalian Ref.22
pTol2-SoNar NADH/NAD+ ratio 420/500 518 Cytosol Zebrafish Ref.22
pcDNA3.1/Hygro(+)-mit-Frex NADH 420/500 518 Mitochondria Mammalian Ref.24
pcDNA3.1/Hygro(+)-cyt-cpYFP pH 420/500 518 Cytosol Mammalian Ref.24
pcDNA3.1/Hygro(+)-mit-cpYFP pH 420/500 518 Mitochondria Mammalian Ref.24
pcDNA3.1/Hygro(+)-cyt-rxRFPa -SH/-SS- 576 600 Cytosol Mammalian Ref.17
pcDNA3.1/Hygro(+)-mit-rxRFP1.1a -SH/-SS- 576 600 Mitochondria Mammalian Ref.43
pcDNA3.1/Hygro(+)-cyt-pHRFPa pH 576 600 Cytosol Mammalian Ref.17
pcDNA3.1/Hygro(+)-mit-pHRFPa pH 576 600 Mitochondria Mammalian Ref.17
pEGFP-N1-cyt-roGFP1 -SH/-SS- 400/490 515 Cytosol Mammalian Ref.10,11
pEGFP-N1-mit-roGFP1 -SH/-SS- 400/490 515 Mitochondria Mammalian Ref.10,11
pLVX-cyt-roGFP1 -SH/-SS- 400/490 515 Cytosol Mammalian Ref.10,11
pcDNA3.1/Hygro(+)-cyt-HyPer H2O2 420/500 516 Cytosol Mammalian Ref.18
pcDNA3.1/Hygro(+)-mit-HyPer H2O2 420/500 516 Mitochondria Mammalian Ref.18
pLVX-cyt-HyPer H2O2 420/500 516 Cytosol Mammalian Ref.18
pcDNA3.1/Hygro(+)-nuc-HyPerRed H2O2 575 605 Nucleus Mammalian Ref.19
pTol2-HyPerRed H2O2 575 605 Cytosol Zebrafish Ref.19

Δ CRITICAL All plasmids are available upon request.

a

The rxRFP, rxRFP1.1 and pHRFP sensors are synthesized according to the cDNA sequences reported by Hui-wang Ai43 (Addgene plasmid #67830, Addgene plasmid #67841, and Addgene plasmid #67832, respectively).