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. 2019 Aug 29;10:276. doi: 10.1186/s13287-019-1378-7

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Cell morphology. a The cells were used to form EBs 3 days after passage. b hES cells formed EBs after suspension culture for 3 days. c The EBs were transferred to standard culture plate for 2 days and some EBs attached. The morphology of EBs attached for 4 (d) and 6 (e) days in EB medium. The cells were stimulated with 1 pM BMP4 in EB medium on days 4 (f) and 6 (g). Scale bar, 300 μm