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. 2019 Aug 28;7:232. doi: 10.1186/s40425-019-0709-7

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Phenotype and functional screening of different tumor infiltrating lymphocytes (TILs) fragments. a Flow cytometry analysis revealed percentages of CD4⁺ and CD8⁺ T cells from TIL-F1 to TIL-F4. b CD4/CD8 ratio. c The percentages of PD-1⁺T cells in four TIL fragments. d Comparision of PD-1 expression. e Comparison of memory-phenotype T cells. f IFN-γ ELISPOT analysis of all four TIL fragments cocultured with autologous tumor cells (ATCs). TILs with no targets are negative controls. Medium well is the blank negative control and OKT-3 well is the positive control. Column histogram summarized the number of positive spots. g IFN-γ ELISA measurement of all four TIL fragments cocultured with ATCs. T cells with no targets are negative controls. The results shown are representative of independent experiment done with repeated 3 times