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. 2019 Jul 12;23(9):5934–5948. doi: 10.1111/jcmm.14402

Figure 5.

Figure 5

miR‐539 promotes the E‐cadherin expression and inhibits expression of DLX1, vimentin, c‐Myc, Smad4, Snail1 and SLUG. A, mRNA expression of DLX1, vimentin, E‐cadherin, c‐Myc, Smad4, Snail1 and SLUG in PC3 cell lines transfected with miR‐539 mimic, miR‐539 inhibitor, si‐DLX1 and miR‐539 inhibitor + si‐DLX1 detected by RT‐qPCR; B, mRNA expression of DLX1, vimentin, E‐cadherin, c‐Myc, Smad4, Snail1 and SLUG in DU145 cell lines transfected with miR‐539 mimic, miR‐539 inhibitor, si‐DLX1 and miR‐539 inhibitor + si‐DLX1 detected by RT‐qPCR; C and E, Western blot analysis of DLX1, vimentin, E‐cadherin, c‐Myc, Smad4, Snail1 and SLUG proteins in PC3 cell lines transfected with miR‐539 mimic, miR‐539 inhibitor, si‐DLX1 and miR‐539 inhibitor + si‐DLX1; D and F, Western blot analysis of DLX1, vimentin, E‐cadherin, c‐Myc, Smad4, Snail1 and SLUG proteins in DU145 cell lines transfected with miR‐539 mimic, miR‐539 inhibitor, si‐DLX1 and miR‐539 inhibitor + si‐DLX1; *P < 0.05, compared with the blank group and the NC group; miR‐539, microRNA‐539; DLX1, distal‐less homeobox 1; NC, negative control; si, small interfering; RT‐qPCR, reverse transcription quantitative polymerase chain reaction