Table 1.

Primer sequences of related genes for reverse transcription quantitative polymerase chain reaction

Gene	Primer sequence (5′‐3′)
β‐actin	Forward: CATATGGCTACATAGGATGATGATATCCCCC
	Reverse: GAATTCACAGAATTCCTAGAAGCATTTGCGGTG
DLX1	Forward: GCGGCCTCTTTGGGACTCACA
	Reverse: GGCCAACGCACTACCCTCCAGA
miR‐539	Forward: GAAGAGGCTAACGTGAGGTTG
	Reverse: CACCATGACCAAGCCACGTAG
c‐myc	Forward: ATGCCCTCAACGTTAGCTTCACC
	Reverse: TTACGCACAAGAGTTCCGTAGCTGTTC
E‐cadherin	Forward: AACGCATTGCCACATACA
	Reverse: GAGCACCTTCCATGACGAC
vimentin	Forward: ACAGGCTTTAGCGAGTTATT
	Reverse: GGGCTCCTAGCGGTTTAG
Smad4	Forward: GGACCGGATTACCCAAGACACA
	Reverse: CTGCAATCGGCATGGTATGAAG
Snail1	Forward: TTCCAGCGCCCTACGACCAG
	Reverse: GCCTTTCCCACTGTCCTCATC
SLUG	Forward: CCTTCCTGGTCAAGAAGCATTTCA
	Reverse: AGGCTCACATATTCCTTGTCACAG
U6	Forward: CTCGCTTCGGCAGCACA
	Reverse: AACGCTTCACGAATTTGCGT

Abbreviations: DLX1, distal‐less homeobox 1; miR‐539, microRNA 539.