Figure 5.

YWPC prevent DOX‐induced cardiac myocyte apoptosis, in vitro. (A) Effects of YWPC (50 mg/L for 24 h) on DOX (5 μmol/L for 24 h)‐induced dissipation of mitochondrial membrane potential measured in H9C2 cells loaded with JC‐1 using fluorescence microscopy. Magnification ×400. (B‐C) Effects of YWPC on DOX‐induced cell apoptosis of H9C2 cells, as detected by Flow cytometry. (D) The relative expression of Bcl‐2, Bax and cleaved caspase‐3 in H9C2 cells with or without YWPC treatment under DOX stimulation. *P < 0.05, vs. the control; ^# P < 0.05 vs. the DOX. DOX, doxorubicin; YWPC, Yellow Wine Polyphenolic Compounds