Figure 1.

NA does not influence homeostatic SLG, but normalizes AA‐induced, excessive, acne‐mimicking lipid production. (A‐B) Nile Red assay. SLG of SZ95 sebocytes was assessed following 48‐hr treatments. Results are expressed in the percentage of the vehicle control (100%, solid line) as mean ± SEM of four independent determinations. Two additional experiments yielded similar results. *, ** and *** mark significant (P < 0.05, 0.01 and 0.001, respectively) differences, as indicated. (C) CyQUANT‐assay. Sebocytes were plated at low (2000/well) initial cell count to enable rapid proliferation. Cell count was assessed following 72‐hr treatments. Results are expressed in the percentage of the 24‐hr vehicle control (100%, solid line) as mean ± SEM of four independent determinations. Two additional experiments yielded similar results. ** marks significant (P < 0.01) differences, as indicated. AA, arachidonic acid; NA, nicotinic acid