Figure 2.

NA does not reduce cytokine expression and release of human sebocytes. (A) Q‐PCR IL‐1α, IL‐1β, IL‐6, IL‐8 and TNF‐α mRNA expressions were determined following 3‐hr LPS‐treatment with or without NA. Data are presented by using ΔΔCT method regarding 18S RNA‐normalized mRNA expressions of the vehicle control as 1 (solid line). Data are expressed as mean ± SEM of 2‐3 determinations. One additional experiment yielded similar results. ** and ***P < 0.01 and 0.001, respectively, as indicated. (B, C) ELISA. IL‐6 and IL‐8 content of the sebocyte supernatants was determined following 3‐hr (B) and 24‐hr (C) LPS‐treatment with or without NA. Data are expressed as mean ± SEM of three determinations. One additional experiment yielded similar results. *, **, and *** mark significant (P < 0.05, 0.01 or 0.001, respectively) differences compared to the vehicle control. LPS, lipopolysaccharide; NA, nicotinic acid