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. 2019 Jul 5;23(9):6203–6214. doi: 10.1111/jcmm.14505

Figure 3.

Figure 3

Lipostatic effects of NA are Ca2+‐dependent ones, and NA increases [Ca2+]IC of human sebocytes in a concentration‐dependent manner, via activating surface membrane non‐TPRV Ca2+‐channels. (A) Nile Red assay. SLG of SZ95 sebocytes was assessed following 48‐hr treatments. Results are expressed in the percentage of the vehicle control (100%, solid line) as mean ± SEM of four independent determinations. One additional experiments yielded similar results. *** marks significant (P < 0.001) difference, as indicated. n.s.: not significant. (B‐C) Fluo‐4 AM‐based fluorescent Ca2+‐measurement. Compounds were applied as indicated by the arrows. Fluorescence (measured in relative fluorescence units) was normalized to the baseline. “Low [Ca2+]EC” indicates the use of nominally Ca2+‐free Hank's solution. One additional experiment yielded similar results. (D) Statistical analysis of the peak fluorescence (F/F0) values shown on panel (C). Data are presented as mean ± SEM of N = 12 independent determinations. ***P < 0.001 compared to the NA treatment in normal [Ca2+]EC. NA: nicotinic acid; RR: ruthenium red (pan‐TRPV channel blocker)