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. 2019 Jul 5;23(9):6203–6214. doi: 10.1111/jcmm.14505

Figure 4.

Figure 4

HCA2 receptor is expressed in cultured human sebocytes. (A) Q‐PCR. Sebocytes were harvested at different confluences. H C A 2 expression was normalized to the level of glyceraldehyde‐3‐phosphate dehydrogenase (GAPDH) of the same sample, and are expressed as mean ± SD of three determinations. (B) Immunofluorescent labelling. HCA2 immunoreactivity was determined by immunofluorescence labelling (Alexa‐Fluor®‐488, green) in SZ95 sebocytes. Nuclei were counterstained by DAPI (blue). NC: negative control. Scale bar: 20 μm