Figure 7.

In vivo implantation of NDNF‐overexpressing old hADSCs increased angiogenesis and arteriole genesis. A, Western blotting analysis of the expression of NDNF protein in the infarcted area of mouse hearts that received implantation of control medium (Medium), empty vector‐transduced old human adipose‐derived stem cells (hADSCs, Old), neuron‐derived neurotrophic factor (NDNF)‐transduced old hADSCs (Old + NDNF) and untransduced young hADSCs (Young). B, NDNF protein levels were significantly higher in the Old + NDNF and Young groups compared with the Old and Medium control groups, n = 3/group. C, Representative micrographs of immunofluorescent staining for von Willebrand factor (vWF, red) with nuclei stained blue with DAPI. D, Capillary density was significantly higher in the Old + NDNF and Young groups compared with the Old and Medium control groups, n = 6/group. E, Representative micrographs of immunofluorescent staining for α‐smooth muscle actin (α‐SMA, green) with nuclei stained blue with DAPI. F, Arteriole density was significantly higher in the Old + NDNF and Young groups compared with the Old and Medium control groups, n = 6/group