Figure 6.

Effect of berberine on LPS‐induced proinflammatory cytokines release in BV2 cells. Berberine did not affect BV2 cell viability at concentrations from 0.5 μmol/L to 5 μmol/L (A). 2.5 μmol/L berberine slightly prevented glutamate‐induced BV2 cell death (B). Berberine decreased LPS‐induced TNF‐ɑ and IL‐1β release by microglia in a dose‐dependent manner, but Go6983 (C,D) and PKC siRNA (E,F) blocked the anti‐neuroinflammatory effect of berberine. All of the results are expressed as the mean ± SD. n = 3; ^# P < 0.05, ^## P < 0.01 vs control;*P < 0.05, **P < 0.01 vs berberine 0 μmol/L; & P < 0.05 vs berberine 1 μmol/L; § P < 0.05 vs berberine 2.5 μmol/L; ※ P < 0.01 vs berberine 5 μmol/L