Figure 7.

Effect of berberine on glutamate‐induced PC12 cells death and the conditioned medium (CM) from LPS/berberine‐treated BV2 cells on PC12 cells death. Berberine did not affect PC12 cell viability at concentrations from 0.5 μmol/L to 15 μmol/L (A). Berberine prevented glutamate‐induced PC12 cell death and Go6983, a PKC inhibitor, blocked the neuroprotective effect of berberine (B). CM from berberine pre‐treatment group attenuated PC12 cell death(C). Original magnification, ×20. All of the results are expressed as the mean ± SD. n = 3; ^# P < 0.05, ^## P < 0.01 vs control;*P < 0.05 vs berberine 0 μmol/L or LPS‐CM group