Figure 3.

Induction of the ISR is critical for resistance to paclitaxel. A, Immunoblots of lysates from blank (without transfected) and EIF2S1 WT (shEIF2S1 + widetype EIF2S1) and EIF2S1 S51A (shEIF2S1 + S51A mutated EIF2S1). B, Indicated cells were incubated with 100 nmol/L paclitaxel for 4 hours and Western blots were performed. C, Indicated cells were incubated with 100 nmol/L paclitaxel and 50 μmol/L Trolox for 48 h. Cell viability was performed by CCK‐8 assay. Percentage of cell survival is represented as mean ± SD from three independent experiments (n = 3, mean ± SD). **P < 0.01, Student's t test. D, Indicated cells stained with Annexin‐V and PI, and analysed by FACS. Bars indicate mean values ± SD of three experiments. **P < 0.01