Figure 1.

The isolation and identification of human adipose derived stem cells (ADSCs). A, The isolation and culture of ADSCs. (I) The primary of ADSCs; (II) The passage 1 of ADSCs; (III) The passage 2 of human ADSCs; (IV) The passage 3 of human ADSCs. B, Flow cytometry analysis of surface phenotypes of ADSCs. C, The histogram of cell surface antigens detected by flow cytometry. D, Adipogenic and osteogenic differentiation of ADSCs in vitro. (b) Oil red‐O staining in ADSCs was positive after cultured in the medium of adipogenic induction (black arrow); (d) Alkaline phosphatase staining in ADSCs was positive after cultured in the medium of osteogenic induction (black arrow); (a and c) negative control