Figure 4.

Identification of Leydig‐like cells derived from induced pluripotent stem cells (ADSC‐LCs) by gene expression assays. A, The detection of expressions of Leydig cell or ADSC relative genes using reverse transcription polymerase chain reaction (RT‐PCR) in ADSCs, LCs and ADSC‐LCs. B, The comparation of expression levels of Leydig cell or ADSC relative genes using qPCR in ADSCs, LCs and ADSC‐LCs. C, The heat map of qPCR results in ADSCs, LCs and ADSC‐LCs (green means low expression level and red represents high expression levels). Mean ± SE, n = 5. *P < 0.05, **P < 0.01, ***P < 0.001 designate significant differences