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. 2019 Jul 5;23(9):6098–6111. doi: 10.1111/jcmm.14476

Figure 1.

Figure 1

LINC01535 interacts with miR‐214. A, Schematic diagram of the predicted miR‐214 binding sites on LINC01535. B, Dual luciferase reporter assay in HeLa cells co‐transfected with luciferase reporter containing LINC01535 or nothing and miR‐214 mimics or miRNA negative control (miR‐NC). Results are displayed as the relative ratio of Firefly luciferase activity to Renilla luciferase activity. C, Dual luciferase reporter assay in HeLa cells co‐transfected with luciferase reporter containing LINC01535 or nothing and miR‐214 inhibitors or inhibitor negative control (inh‐NC). Results are displayed as the relative ratio of Firefly luciferase activity to Renilla luciferase activity. D, The levels of LINC01535 in cytoplasmic or nuclear RNAs purified from Huh7 cells. U6 and GAPDH serve as nuclear and cytoplasmic control, respectively. E, HeLa cell lysates were incubated with biotin‐labeled LINC01535; after pull‐down, miRNAs was extracted and detected by qRT‐PCR. Results are shown as mean ± SD of three independent experiments. **P < 0.01, ***P < 0.001, ns, not significant, by Student's t test